主要功能

本系統(tǒng)是全球檢出限和靈敏度很高的乙烯監(jiān)測系統(tǒng)，主要用于植物研究相關的乙烯氣體監(jiān)測，如種子發(fā)芽、植物生長發(fā)育、開花生理、植物器官衰老、基因表達、植物病原體相互作用、植物激素間相互作用、蔬果收貨后保藏、植物抗逆性研究（干旱、高溫、重金屬）等。

其中乙烯氣體檢測儀 ETD-300 采用*的激光技術（光聲學原理），即樣品乙烯在光聲腔吸收激光后釋放熱使光聲腔內部產生壓力，隨激光頻率增減形成能被微型麥克風檢測到的壓力差，而乙烯濃度越高壓力差越大，從而據聲波強度差可實時快速測量乙烯氣體（C2H4）絕對濃度；閥門控制箱 VC-6 全自動化和電腦控制，接一個即可以使單個氣體檢測儀實現(xiàn)6個樣品的自動切換測量，單個乙烯氣體檢測儀可以接一個或多個閥門控制箱；烴分解器 CAT-1 則利用鉑金顆粒催化烴氧化分解為水蒸氣和 CO2，為系統(tǒng)提供無烴干擾的樣品空氣。

測量參數

測量參數：乙烯濃度（ppbv）、氣體流速（l/h）、背景值、模擬輸入（V）

計算參數：乙烯產量（nl/h）

連續(xù)流動測定（左）和積累測定（右）的乙烯監(jiān)測數據圖

應用領域

用于環(huán)境、醫(yī)學、農業(yè)、工業(yè)、生態(tài)、生物等監(jiān)測領域。特別適合植物生理、發(fā)育研究的超靈敏乙烯測量。

主要技術參數

產地：荷蘭Sensor Sense  

應用舉例

1.1 乙烯測定在高溫脅迫研究中的應用舉例

實驗內容簡介：以生長 3 周的擬南芥野生型 Col-0，突變體 NahG 和 opr3 植株為材料，研究了其高溫脅迫下的乙烯釋放。其中，野生型 Col-0 高溫脅迫（38℃）下，電導率（電解質滲透率）、水楊酸和茉莉酸含量和乙烯釋放增加；突變體 NahG 和 opr3 高溫脅迫（38℃）下電導率、茉莉酸和乙烯釋放也增加，但都低于野生型 Col-0，而高溫脅迫后恢復階段（水中 22℃）電導率明顯高于 Col-0。研究結果表明：高溫脅迫下，乙烯迅速產生，其生產受到茉莉酸和水楊酸的調控。總的來說，茉莉酸與水楊酸協(xié)同調節(jié)植物對高溫脅迫的耐受，而乙烯主要加快細胞死亡；突變體 NahG 和 opr3 比野生型 Col-0 的耐熱性差，細胞死亡多。

圖1 高溫處理下擬南芥植株的水楊酸（a）、電導率（b、c）和乙烯釋放（d、e）

WT：擬南芥野生型；突變株opr3 ；突變株NahG以及培養(yǎng)基agar

Clarke, S.M., et al., Jasmonates act with salicyli c acid to confer basal thermotolerance in Arabidopsis thaliana. New Phytologist, 2009. 182(1): p. 175-187.

1.2 乙烯測定在病菌感染研究中的應用舉例

實驗內容簡介：以品種為 Money Maker 和 Daniela 的成熟番茄果實為材料，研究了其感染番茄灰霉病菌株 VTF1 的乙烯釋放?；颐共【梢栽隗w外產生乙烯，其乙烯釋放與其說與分生孢子萌發(fā)相關，不如說與菌絲生長更相關，且分生孢子濃度越大真菌的乙烯釋放越多。感染灰霉病的兩種番茄的乙烯釋放規(guī)律與灰霉病菌類似；但釋放量是其 100 倍。結合受感染番茄的細胞學參數，研究結果表明：番茄-真菌系統(tǒng)的乙烯釋放不是由番茄灰霉病菌引起的，雖說與其內部的真菌生長速率十分同步。

圖 2 真菌（160 μl 懸浮液）的乙烯產量

● 1.5*108 灰霉病菌分生孢子 ml-1  ▲ 2*107 灰霉病菌分生孢子 ml-1  ■ 2*105 灰霉病菌分生孢子 ml-1

圖3  模擬感染和不同濃度番茄灰霉病菌感染的兩種番茄的乙烯釋放

A.番茄品種 Money Maker；B.番茄品種 Daniela；

○ 模擬番茄灰霉病菌感染  ● 1.5*108 灰霉病菌分生孢子 ml-1  ▲ 2*107 灰霉病菌分生孢子 ml-1  ■ 2*105 灰霉病菌分生孢子 ml-1

Cristescu, S.M., et al., Ethylene Production by Botrytis cinerea In Vitro and in Tomatoes. Applied and Environmental Microbiology, 2002. 68 (11): p. 5342-5350.

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